The enabling absorption (ENA) device is a new in vitro tool that captures the complex dynamics between digestion and absorption in the small intestine. Cell-based systems (e.g. Caco-2) as well as artificial membranes can be used to mimic the intestinal wall to predict drug permeation into the serosal compartment. In addition, this assay allows for the evaluation of (i) drug concentrations in the aqueous, oil and solid phase in the digestion compartment and, (ii) the extent of digestion. Donor-volume-to-absorption-ratios that resemble the small intestine are used.
In vitro lipolysis assays are used to evaluate the extent of digestion and aqueous drug concentration in simulated intestinal fluids.
In vitro lipolysis
Cell-based systems as well as artificial membrane are used to evaluate drug permeation in the small intestine.